Regeneration of Transgenic Soybean (Glycine max) Plants from Electroporated Protoplasts
نویسندگان
چکیده
منابع مشابه
Soybean [Glycine max (L.) Merr].
In this chapter we describe an Agrobacterium tumefaciens transformation method of soybean that utilizes mature half seeds and regeneration from the cotyledonary node region. This method results in fertile transformed soybean plants and transgenic seed in approximately 9 months. Using mature half seeds as starting material has proven to be a reliable method that does not require additional wound...
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Using precise pollen species determination by conventional microscopic methods, accompanied by molecular genetic markers, we found bees collect GMO (genetically modified) soybean pollen and incorporate it in Yucatan honey. Honey comb samples from Las Flores, Campeche, Mexico, often contained soybean pollen. Pollen in honey was analyzed in nine samples; six contained substantial soy pollen and t...
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A fatty acid hydroperoxidelyase (HPL) enzyme from soybean (Glycine max) seeds was purified by ammonium sulphate precipitation followed by ion exchange chromatography on DEAE-Cellulose and DEAE-Sephacel. HPL showed specific activity of 161U/mg with fold purification of 16.4 and enzyme yield was found to be 9.06%. The purified enzyme on SDSPAGE showed a prominent band of ~53KDa. Kinetic studies w...
متن کاملScreening of Microfungi from Soybean (glycine Max L.) Seeds
Seed health testing by studying the seed-borne fungi of soybean seeds is an important step in the management of the seed-borne diseases. In the present study seed-borne fungi was studied by using agar plate method. A number of seed-borne fungal species was isolated from the agar plate. These seed-borne fungal sps. were Aspergillus niger, A. flavus, A. fumigatus, Chaetomium globosum, Curvularia ...
متن کاملPurification and Properties of Arginase from Soybean, Glycine max, Axes.
Arginase (EC 3.5.3.1) was purified to homogeneity from cytosol of soybean, Glycine max, axes by chromatographic separations on Sephadex G-200, DEAE-sephacel, hydroxyapatite, and arginine-affinity columns. The molecular weight of the enzyme estimated by pore gradient gel electrophoresis was 240,000, while sodium dodecyl sulfate polyacrylamide gel electrophoresis gave a single band at the molecul...
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ژورنال
عنوان ژورنال: PLANT PHYSIOLOGY
سال: 1992
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.99.1.81